Confluence Retirement

In an effort to consolidate USGS hosted Wikis, the myUSGS Confluence service is targeted for retirement on January 28, 2022. The official USGS Wiki and collaboration space is now SharePoint. Please migrate existing spaces and content to the SharePoint platform and remove it from Confluence at your earliest convenience. If you need any additional information or have any concerns about this change, please contact myusgs@usgs.gov. Thank you for your prompt attention to this matter.
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  1. Gblocks- For those of you who use Gblocks as controls or standards in ddPCR, is there a certain length of time you use them once hydrated? Do you keep using them until they seem to have issues or hydrate a new tube even though the current one may be good if say it's been longer than 3/6 months? Do you only freeze/thaw the original stock a certain number of times? We use them a lot in our assays and typically they work really well, until one day they just don't. Then I go through troubleshooting with everything pointing to the Gblocks just "running out of steam." Just curious to hear some thoughts or if you can pinpoint a length of time they usually turn "bad" on you. Thanks